The Porcine Apolipoprotein A4 (APOA4) ELISA Kit from Assay Genie is a powerful tool for accurately measuring levels of APOA4 in porcine serum, plasma, and tissue samples. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a variety of research applications.APOA4 is a key protein in lipid metabolism and is known to play a crucial role in regulating cholesterol levels and lipid transport in the body.
Dysregulation of APOA4 has been associated with various metabolic disorders, making it a valuable biomarker for studying conditions such as obesity, diabetes, and cardiovascular diseases.Whether you are conducting basic research or clinical studies, the Porcine APOA4 ELISA Kit offers a convenient and efficient way to analyze APOA4 levels in porcine samples, helping you gain valuable insights into the role of this important protein in health and disease.
Product Name:
Porcine Apolipoprotein A4 (APOA4) ELISA Kit
Product Code:
PRDL00014
Size:
96 Assays
Target:
APOA4
Synonyms:
ApoA-IV, ApoAIV, Apo-A4
Detection Method:
Sandwich
Range:
6.25-400ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
