The PP2A-Abeta/PR65beta/PPP2R1B Polyclonal Antibody (CAB24836) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. It binds to the PR65 subunit of PP2A, allowing for the detection and analysis of this critical protein in various cell types.PP2A is a key phosphatase involved in the regulation of cellular processes, including cell growth, division, and signaling. The PR65 subunit is essential for the structure and function of the PP2A holoenzyme, making it a critical target for research in the fields of cell biology, cancer biology, and drug development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
PP2A-Abeta/PR65beta/PPP2R1B Polyclonal Antibody
SKU:
CAB24836
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PPP2R1B, PP2A-Abeta, PR65B, protein phosphatase 2 scaffold subunit Abeta, PP2A-Aβ/PR65β/PPP2R1B
Positive Sample:
Rat spleen
Cellular Localization:
Cytoplasm.
Calculated MW:
52kDa/61kDa/66kDa/73kDa
Observed MW:
66kDa
This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
5519
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat spleen using PP2A-Aβ/PR65β/PPP2R1B Rabbit pAb (CAB24836) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
