The PP2A-B56beta/PR61beta/PPP2R5B Polyclonal Antibody (CAB19332) is a high-quality antibody developed for reliable detection and analysis of target proteins. PPP2R5B is a regulatory subunit of Protein Phosphatase 2A (PP2A), one of the four major serine/threonine phosphatases implicated in the negative control of cell growth and division. It belongs to the B56 subfamily and encodes the beta isoform, modulating the substrate selectivity and catalytic activity of the PP2A holoenzyme by associating with its core catalytic and regulatory subunits.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
PP2A-B56beta/PR61beta/PPP2R5B Polyclonal Antibody
SKU:
CAB19332
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
B56B, PR61B, B56beta, PP2A-B56β/PR61β/PPP2R5B
Positive Sample:
Rat brain
Cellular Localization:
Cytoplasm.
Calculated MW:
57kDa
Observed MW:
57kDa
The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B56 subfamily.
Purification Method
Affinity purification
Gene ID
5526
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat brain using PP2A-B56β/PR61β/PPP2R5B Rabbit pAb (CAB19332) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
