The PP2A-Aalpha/PR65alpha/PPP2R1A Antibody (CAB5799) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications including Western blot, immunohistochemistry, and immunofluorescence.PPP2R1A plays a crucial role in the regulation of cell growth, proliferation, and differentiation by dephosphorylating target proteins. Dysregulation of PPP2R1A has been implicated in various diseases including cancer, neurodegenerative disorders, and metabolic conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PP2A-Aalpha/PR65alpha/PPP2R1A Antibody
SKU:
CAB5799
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
MCF7, HT-29, Mouse liver, Mouse brain, Mouse kidney, Rat liver, Rat brain
Cellular Localization:
Chromosome, Cytoplasm, Centromere.
Calculated MW:
65kDa
Observed MW:
65kDa
This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
5518
RRID
AB_2766551
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PP2A-Aα/PR65α/PPP2R1A Rabbit pAb (CAB5799) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of U2OS cells using PP2A-Aα/PR65α/PPP2R1A Rabbit pAb (CAB5799). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
