Anti-PPP2R5C Antibody (CAB5480)

Antibody Name:	Anti-PPP2R5C Antibody
Antibody SKU:	CAB5480
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human PPP2R5C (NP_848702.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, K-562, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human PPP2R5C (NP_848702.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLTC NKAG SRMV VDAA NSNG PFQP VVLL HIRD VPPA DQEK LFIQ KLRQ CCVL FDFV SDPL SDLK WKEV KRAA LSEM VEYI THNR NVIT EPIY PEVV HMFA VNMF RTLP PSSN PTGA EFDP EEDE PTLE AAWP HLQL VYEF FLRF LESP DFQP NIAK KYID QKFV LQLL ELFD SEDP RERD FLKT TLHR IYGK FLGL RAYI RKQI NNIF YRFI YETE HHNG IAEL LEIL GSII NGFA LPLK EEHK IFLL KVLL PLHK VKSL SVYH PQLA YCVV QFLE KDST LTEP VVMA LLKY WPKT HSPK EVMF LNEL EEIL DVIE PSEF
Gene ID:	5527
Uniprot:	Q13362
Cellular Location:	Chromosome, Nucleus, centromere
Calculated MW:	52kDa/56kDa/61kDa/62kDa/64kDa
Observed MW:	68-71kDa

Synonyms:

PPP2R5C, B56G, PR61G, B56gamma

Background:

The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	PPP2R5C: a regulatory subunit of PP2A that might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.Three alternatively spliced human isoforms have been described.
UniProt Protein Details:	Protein type:Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 14q32.31 Cellular Component: protein phosphatase type 2A complex; nucleus; chromosome, pericentric region Molecular Function:protein binding; protein phosphatase type 2A regulator activity Biological Process: negative regulation of cell proliferation; proteasomal ubiquitin-dependent protein catabolic process; regulation of catalytic activity; signal transduction; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis
NCBI Summary:	The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13362
NCBI GenInfo Identifier:	116241235
NCBI Gene ID:	5527
NCBI Accession:	Q13362.3
UniProt Secondary Accession:	Q13362,Q14391, Q15060, Q15174, Q6ZN33, B4DYJ8, B5BUA5 F5GWP3,
UniProt Related Accession:	Q13362
Molecular Weight:	524
NCBI Full Name:	Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
NCBI Synonym Full Names:	protein phosphatase 2, regulatory subunit B', gamma
NCBI Official Symbol:	PPP2R5C
NCBI Official Synonym Symbols:	B56G; PR61G
NCBI Protein Information:	serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform; B' alpha regulatory subunit; PP2A B subunit isoform B'-gamma; PP2A B subunit isoform R5-gamma; PP2A B subunit isoform B56-gamma; PP2A B subunit isoform PR61-gamma; renal carcinoma antigen NY-REN-29; protein phosphatase 2, regulatory subunit B', gamma isoform; protein phosphatase 2, regulatory subunit B (B56), gamma isoform
UniProt Protein Name:	Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
UniProt Synonym Protein Names:	PP2A B subunit isoform B'-gamma; PP2A B subunit isoform B56-gamma; PP2A B subunit isoform PR61-gamma; PP2A B subunit isoform R5-gamma; Renal carcinoma antigen NY-REN-29
Protein Family:	Serine/threonine-protein phosphatase
UniProt Gene Name:	PPP2R5C
UniProt Entry Name:	2A5G_HUMAN

	Western blot analysis of extracts of various cell lines, using PPP2R5C antibody (CAB5480) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat heart using PPP2R5C antibody (CAB5480) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using PPP2R5C antibody (CAB5480) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using PPP2R5C antibody (CAB5480) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)