Anti-PSMB3 Antibody (CAB9947)

Antibody Name:	Anti-PSMB3 Antibody
Antibody SKU:	CAB9947
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human PSMB3 (NP_002786.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	NIH/3T3, MCF7, HeLa, Jurkat, Mouse brain, Rat liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human PSMB3 (NP_002786.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSIM SYNG GAVM AMKG KNCV AIAA DRRF GIQA QMVT TDFQ KIFP MGDR LYIG LAGL ATDV QTVA QRLK FRLN LYEL KEGR QIKP YTLM SMVA NLLY EKRF GPYY TEPV IAGL DPKT FKPF ICSL DLIG CPMV TDDF VVSG TCAE QMYG MCES LWEP NMDP DHLF ETIS QAML NAVD RDAV SGMG VIVH IIEK DKIT TRTL KARM D
Gene ID:	5691
Uniprot:	P49720
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	22kDa
Observed MW:	23kDa

Synonyms:

PSMB3, HC10-II

Background:

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The 26 S proteasome may be involved in trinucleotide repeat expansion, a phenomenon which is associated with many hereditary neurological diseases. Pseudogenes have been identified on chromosomes 2 and 12. Alternative splicing results in multiple transcript variants

UniProt Protein Function:	PSMB3: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Belongs to the peptidase T1B family.
UniProt Protein Details:	Protein type:Cell cycle regulation; EC 3.4.25.1; Protease; Proteasome complex Chromosomal Location of Human Ortholog: 17q12 Cellular Component: cytosol; nucleoplasm; nucleus; proteasome complex; proteasome core complex Molecular Function:protein binding Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; transmembrane transport; tumor necrosis factor-mediated signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway
NCBI Summary:	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The 26 S proteasome may be involved in trinucleotide repeat expansion, a phenomenon which is associated with many hereditary neurological diseases. Pseudogenes have been identified on chromosomes 2 and 12. Alternative splicing results in multiple transcript variants [provided by RefSeq, Sep 2013]
UniProt Code:	P49720
NCBI GenInfo Identifier:	20532411
NCBI Gene ID:	5691
NCBI Accession:	P49720.2
UniProt Secondary Accession:	P49720,P31147, Q0P6J7, Q96E27,
UniProt Related Accession:	P49720
Molecular Weight:	23kDa
NCBI Full Name:	Proteasome subunit beta type-3
NCBI Synonym Full Names:	proteasome subunit beta 3
NCBI Official Symbol:	PSMB3
NCBI Official Synonym Symbols:	HC10-II
NCBI Protein Information:	proteasome subunit beta type-3
UniProt Protein Name:	Proteasome subunit beta type-3
UniProt Synonym Protein Names:	Proteasome chain 13; Proteasome component C10-II; Proteasome theta chain
Protein Family:	Proteasome
UniProt Gene Name:	PSMB3

	Western blot analysis of extracts of various cell lines, using PSMB3 antibody (CAB9947) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using PSMB3 antibody (CAB9947) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using PSMB3 antibody (CAB9947) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using PSMB3 antibody (CAB9947) at dilution of 1:100 (40x lens).

Antibodies

PSMB3 Antibody (PACO11533)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)