Anti-PTK7 Antibody (CAB9839)

Antibody Name:	Anti-PTK7 Antibody
Antibody SKU:	CAB9839
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 811-1070 of human PTK7 (NP_002812.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460, HepG2, SKOV3, Jurkat, Mouse lung, Mouse liver, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 811-1070 of human PTK7 (NP_002812.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FLAK AQGL EEGV AETL VLVK SLQS KDEQ QQLD FRRE LEMF GKLN HANV VRLL GLCR EAEP HYMV LEYV DLGD LKQF LRIS KSKD EKLK SQPL STKQ KVAL CTQV ALGM EHLS NNRF VHKD LAAR NCLV SAQR QVKV SALG LSKD VYNS EYYH FRQA WVPL RWMS PEAI LEGD FSTK SDVW AFGV LMWE VFTH GEMP HGGQ ADDE VLAD LQAG KARL PQPE GCPS KLYR LMQR CWAL SPKD RPSF SEIA SALG DSTV DSKP
Gene ID:	5754
Uniprot:	Q13308
Cellular Location:	Cell junction, Membrane, Single-pass type I membrane protein
Calculated MW:	89kDa/103kDa/112kDa/113kDa/118kDa/119kDa
Observed MW:	80kDa

Synonyms:

PTK7, CCK-4, CCK4

Background:

This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
NCBI Summary:	This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:	Q13308
NCBI GenInfo Identifier:	116242736
NCBI Gene ID:	5754
NCBI Accession:	Q13308.2
UniProt Secondary Accession:	Q13308,Q13417, Q5T650, Q6IQ54, Q8NFA5, Q8NFA6, Q8NFA7 Q8NFA8, A8K974, B7Z477, E9PFZ5,
UniProt Related Accession:	Q13308
Molecular Weight:	119,197 Da
NCBI Full Name:	Inactive tyrosine-protein kinase 7
NCBI Synonym Full Names:	protein tyrosine kinase 7 (inactive)
NCBI Official Symbol:	PTK7
NCBI Official Synonym Symbols:	CCK4; CCK-4
NCBI Protein Information:	inactive tyrosine-protein kinase 7
UniProt Protein Name:	Inactive tyrosine-protein kinase 7
UniProt Synonym Protein Names:	Colon carcinoma kinase 4; CCK-4; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; Tyrosine-protein kinase-like 7
Protein Family:	Inactive tyrosine-protein kinase
UniProt Gene Name:	PTK7
UniProt Entry Name:	PTK7_HUMAN

	Western blot analysis of extracts of various cell lines, using PTK7 antibody (CAB9839) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded mouse brain using PTK7 antibody (CAB9839) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using PTK7 antibody (CAB9839) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using PTK7 antibody (CAB9839) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)