Anti-PTP4A3 Antibody (CAB2004)

Antibody Name:	Anti-PTP4A3 Antibody
Antibody SKU:	CAB2004
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human PTP4A3 (NP_009010.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	K-562, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human PTP4A3 (NP_009010.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MARM NRPA PVEV SYKH MRFL ITHN PTNA TLST FIED LKKY GATT VVRV CEVT YDKT PLEK DGIT VVDW PFDD GAPP PGKV VEDW LSLV KAKF CEAP GSCV AVHC VAGL GRKR RGAI NSKQ LTYL EKYR PKQR LRFK DPHT HKTR CCVM
Gene ID:	11156
Uniprot:	O75365
Cellular Location:	Cell membrane, Early endosome
Calculated MW:	10kDa/16kDa/19kDa
Observed MW:	15kDa

Synonyms:

PTP4A3, PRL-3, PRL-R, PRL3

Background:

This gene encodes a member of the protein-tyrosine phosphatase family. Protein tyrosine phosphatases are cell signaling molecules that play regulatory roles in a variety of cellular processes. Studies of this class of protein tyrosine phosphatase in mice demonstrates that they are prenylated in vivo, suggesting their association with cell plasma membrane. The encoded protein may enhance cell proliferation, and overexpression of this gene has been implicated in tumor metastasis. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	PTP4A3: Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis. May be involved in the progression of cardiac hypertrophy by inhibiting intracellular calcium mobilization in response to angiotensin II. Belongs to the protein-tyrosine phosphatase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor) Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: early endosome; plasma membrane Molecular Function:prenylated protein tyrosine phosphatase activity Biological Process: endothelial cell migration
NCBI Summary:	This gene encodes a member of the protein-tyrosine phosphatase family. Protein tyrosine phosphatases are cell signaling molecules that play regulatory roles in a variety of cellular processes. Studies of this class of protein tyrosine phosphatase in mice demonstrates that they are prenylated in vivo, suggesting their association with cell plasma membrane. The encoded protein may enhance cell proliferation, and overexpression of this gene has been implicated in tumor metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Code:	O75365
NCBI GenInfo Identifier:	68566154
NCBI Gene ID:	11156
NCBI Accession:	O75365.2
UniProt Secondary Accession:	O75365,Q8IVN5, Q99849, Q9BTW5,
UniProt Related Accession:	O75365
Molecular Weight:	173
NCBI Full Name:	Protein tyrosine phosphatase type IVA 3
NCBI Synonym Full Names:	protein tyrosine phosphatase type IVA, member 3
NCBI Official Symbol:	PTP4A3
NCBI Official Synonym Symbols:	PRL3; PRL-3; PRL-R
NCBI Protein Information:	protein tyrosine phosphatase type IVA 3; protein-tyrosine phosphatase 4a3; potentially prenylated protein tyrosine phosphatase; protein-tyrosine phosphatase of regenerating liver 3
UniProt Protein Name:	Protein tyrosine phosphatase type IVA 3
UniProt Synonym Protein Names:	PRL-R; Protein-tyrosine phosphatase 4a3; Protein-tyrosine phosphatase of regenerating liver 3; PRL-3
UniProt Gene Name:	PTP4A3
UniProt Entry Name:	TP4A3_HUMAN

	Western blot analysis of extracts of various cell lines, using PTP4A3 antibody (CAB2004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human gastric cancer using PTP4A3 antibody (CAB2004) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)