Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery.
Assay Genie's pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at Ex/Em = 530/585nm is directly proportional to pyruvate concentration in the sample.Description
Pyruvate Assay Kit
Applications
For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.
Key Features
- Sensitive and accurate. Use as little as 10 uL samples. Linear detection range in 96-well plate: 2 to 500 uM (17 ug/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 uM for fluorimetric assays.
- Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays.
- Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
Pyruvate Assay Kit Information:
| Kit Contents | Description |
| Kit Includes: | Enzyme Mix: 10 mL Dye Reagent: 120 mL Standard: 400 mL 25 mM Pyruvate |
| Kit Requires: | Pipeting devices, centrifuge tubes, Clear flat-bottom 96-well plates, black 96-well or 384-well plates (e.g. Corning Costar) and plate reader |
| Method of Detection: | OD570nm, or FL530/585nm |
| Detection Limit: | OD, FL: 2, 0.2 uM |
| Samples: | Biological |
| Species: | All |
| Protocol Length: | 20 min |
| Size: | 100 tests |
| Storage: | Store all reagents at -20°C |
| Shelf Life: | 6 months |
