The QuickStep Chicken CORT (Corticosterone) ELISA Kit is a highly sensitive and specific assay designed for the precise measurement of corticosterone levels in chicken serum, plasma, and tissue samples. This kit provides reliable and reproducible results, making it ideal for a variety of research applications in the field of avian endocrinology.Corticosterone is a vital steroid hormone that plays a crucial role in the regulation of stress response, metabolism, and immune function in chickens. Monitoring corticosterone levels is essential for studying the physiological responses to stress, reproduction, and environmental changes in poultry research.
With the QuickStep Chicken CORT ELISA Kit, researchers can accurately quantify corticosterone levels in chicken samples, allowing for a better understanding of the impact of stress and other factors on poultry health and welfare. This kit is a valuable tool for studying the effects of various stressors on chickens and developing strategies to improve their well-being.
Product Name:
QuickStep Chicken Corticosterone ELISA Kit
Product Code:
QSES011
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Chicken
Sensitivity:
1.89 ng/mL
Range:
3.13-200 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Corticosterone
Application:
This ELISA kit applies to the in vitro quantitative determination of Cort concentrations in serum and plasma.
Specificity:
This kit recognizes Cort in samples. No significant cross-reactivity or interference between Cort and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted or 2~8 fold diluted Chicken serum, plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Bovine Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
10.37
31.21
72.60
10.56
29.42
71.81
Standard deviation
0.61
1.49
2.48
0.73
1.61
2.50
CV (%)
5.84
4.77
3.41
6.95
5.46
3.48
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
88-102
95
EDTA plasma(n=8)
92-104
98
Samples were spiked with high concentrations of Chicken Cort and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
