Anti-RAMP3 Antibody (CAB6715)

Antibody Name:	Anti-RAMP3 Antibody
Antibody SKU:	CAB6715
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human RAMP3 (NP_005847.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, SW620, Mouse brain, Mouse skin, Mouse kidney, Mouse heart, Mouse eye, Rat brain, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human RAMP3 (NP_005847.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	METG ALRR PQLL PLLL LLCG GCPR AGGC NETG MLER LPLC GKAF ADMM GKVD VWKW CNLS EFIV YYES FTNC TEME ANVV GCYW PNPL AQGF ITGI HRQF FSNC TVDR VHLE DPPD EV
Gene ID:	10268
Uniprot:	O60896
Cellular Location:	Cell membrane, Membrane, Single-pass type I membrane protein
Calculated MW:	16kDa
Observed MW:	40kDa

Synonyms:

RAMP3

Background:

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP3) protein, CRLR functions as an adrenomedullin receptor.

UniProt Protein Function:	RAMP3: Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for adrenomedullin (AM) together with CALCRL. Belongs to the RAMP family.
UniProt Protein Details:	Protein type:Receptor, misc.; Membrane protein, integral Chromosomal Location of Human Ortholog: 7p13-p12 Cellular Component: cell surface; integral to plasma membrane; lysosome; plasma membrane; intracellular; receptor complex Molecular Function:protein binding; coreceptor activity; receptor activity; protein transporter activity Biological Process: intracellular protein transport; protein transport; receptor internalization; calcium ion transport; negative regulation of transcription, DNA-dependent; positive regulation of receptor recycling; regulation of G-protein coupled receptor protein signaling pathway
NCBI Summary:	The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP3) protein, CRLR functions as an adrenomedullin receptor. [provided by RefSeq, Jul 2008]
UniProt Code:	O60896
NCBI GenInfo Identifier:	11134424
NCBI Gene ID:	10268
NCBI Accession:	O60896.1
UniProt Secondary Accession:	O60896,Q7Z2Y1,
UniProt Related Accession:	O60896
Molecular Weight:	148
NCBI Full Name:	Receptor activity-modifying protein 3
NCBI Synonym Full Names:	receptor (G protein-coupled) activity modifying protein 3
NCBI Official Symbol:	RAMP3
NCBI Protein Information:	receptor activity-modifying protein 3; CRLR activity-modifying protein 3; receptor activity modifying protein 3; receptor-activity-modifying protein 3; receptor (calcitonin) activity modifying protein 3; calcitonin receptor-like receptor activity modifyin
UniProt Protein Name:	Receptor activity-modifying protein 3
UniProt Synonym Protein Names:	Calcitonin-receptor-like receptor activity-modifying protein 3; CRLR activity-modifying protein 3
Protein Family:	Receptor activity-modifying protein
UniProt Gene Name:	RAMP3
UniProt Entry Name:	RAMP3_HUMAN

	Western blot analysis of extracts of various cell lines, using RAMP3 antibody (CAB6715) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of MCF7 cells using RAMP3 antibody (CAB6715). Blue: DAPI for nuclear staining.

Antibodies

RAMP3 Antibody (PACO11706)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)