Description
Rat AKI Array (7 targets) (SARB0099)
The Rat AKI Array (7 Targets) - SARb0099 is a specialized biological assay kit designed for researchers studying acute kidney injury in rats. This array contains seven unique targets crucial for investigating the molecular mechanisms involved in AKI, including markers of inflammation, oxidative stress, and cell damage.The kit allows for simultaneous detection and quantification of these targets in a single experiment, providing a comprehensive profile of kidney injury progression. With high sensitivity and specificity, researchers can confidently analyze changes in protein expression levels in response to different treatment interventions or disease models.
Pre-validated for rat samples, the Rat AKI Array (7 Targets) - SARb0099 is a valuable tool for studying AKI pathophysiology and developing new therapeutic strategies. By gaining insights into the key signaling pathways and biomarkers associated with AKI, researchers can further our understanding of this complex condition and potentially identify novel targets for intervention.
| Product Sku: | SARB0099 |
| Size: | 2, 4, or 8 |
| Species Detected: | Rat |
| Number of Targets Detected: | 7 |
| Gene Symbols: | CCL2, CST3, FABP1, HAVCR1, LCN2, TIMP1, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
TIM-1 (KIM-1)
| FABP1
| MCP-1 (CCL2)
| Cystatin C
| Lipocalin-2 (NGAL)
|
TIMP-1
| VEGF-A
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Rat Acute Kidney Injury (AKI) Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
