Description
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Rat Cytokine Array (34 targets) (SARB0102)
The Rat Cytokine Array (SARB0102) is a powerful tool for researchers studying cytokine signaling in rat samples. This array includes 34 targets, allowing for a comprehensive analysis of cytokine expression levels in a single experiment.With a focus on immune regulation and inflammation, this cytokine array is ideal for researchers studying various disease models, including cancer, autoimmune disorders, and chronic inflammatory conditions. By simultaneously measuring multiple cytokines in rat samples, researchers can gain valuable insights into the immune response and potential therapeutic targets.
Designed for ease of use and high sensitivity, the Rat Cytokine Array (SARB0102) provides reliable and reproducible results for a wide range of experimental applications. Whether investigating the impact of drug treatments, disease progression, or immune modulation, this array offers a comprehensive solution for researchers seeking to uncover the complex interactions of cytokines in rat models.
| Product Sku: | SARB0102 |
| Size: | 2, 4, or 8 |
| Species Detected: | Rat |
| Number of Targets Detected: | 34 |
| Gene Symbols: | AGER, AGRN, CCL2, CCL20, CD86, CNTF, CSF2, CX3CL1, CXCL1, CXCL2|CXCL3, CXCL5, FASLG, ICAM1, IFNG, IL10, IL13, IL1A, IL1B, IL1RL2, IL2, IL4, IL6, INHBA, LEP, MMP8, NGF, PDGFA, PPBP, PRLR, SELL, TIMP1, TNF, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Activin A
| Agrin
| CD86 (B7-2)
| beta-NGF
| CINC-1 (CXCL1)
|
CINC-2
| CINC-3
| CNTF
| Fas Ligand (TNFSF6)
| Fractalkine (CX3CL1)
|
GM-CSF
| ICAM-1 (CD54)
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
|
IL-1 R6 (IL-1 Rrp2)
| IL-2
| IL-4
| IL-6
| IL-10
|
IL-13
| Leptin
| LIX
| L-Selectin (CD62L)
| MCP-1 (CCL2)
|
MIP-3 alpha (CCL20)
| MMP-8
| PDGF-AA
| Prolactin R
| RAGE
|
TCK-1 (CXCL7)
| TIMP-1
| TNF alpha
| VEGF-A
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Rat Cytokine Antibody Array C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
