Description
Rat Growth Factor Array (14 targets) (SARB0103)
The Rat Growth Factor Array (SARB0103) is a powerful research tool designed for studying the expression of 14 different growth factors in rat samples. This array allows for the simultaneous detection and quantification of various growth factors, providing valuable insights into the regulation of cell growth, differentiation, and proliferation.With targets including important growth factors such as EGF, FGF-2, VEGF, and PDGF-BB, this array offers researchers a comprehensive overview of the molecular factors involved in rat cell growth and development. By utilizing this array, researchers can evaluate the levels of multiple growth factors in a single experiment, saving time and resources while generating accurate and reliable data.
The Rat Growth Factor Array is a high-quality and reliable research tool that is ideal for studies in developmental biology, tissue regeneration, and cancer research. Its ease of use and comprehensive coverage of key growth factors make it a valuable asset for researchers looking to gain a deeper understanding of the molecular mechanisms underlying cell growth and differentiation in rat models.
| Product Sku: | SARB0103 |
| Size: | 2, 4, or 8 |
| Species Detected: | Rat |
| Number of Targets Detected: | 14 |
| Gene Symbols: | BDNF, CSF2, EGF, FGF2, FLT3LG, GDNF, HGF, IGF1, IGFBP5, INHBA|NGF, PDGFA, PDGFB, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Activin A
| BDNF
| beta-NGF
| bFGF
| EGF
|
Flt-3 Ligand
| GDNF
| GM-CSF
| HGF
| IGF-1
|
PDGF-AA
| PDGF-BB
| IGFBP-5
| VEGF-A
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Rat Growth Factor Array 1
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
