The Recombinant ACE2 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C120023) against ACE2. The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC-P and IP applications. It has been validated by WB analysis of MCF-7, Mouse kindey, Mouse lung, Mouse heart, Rat heart and IHC analysis of Mouse kidney. The target is localized to the secreted, cell membrane, cytoplasm, cell projection, cilium, apical cell membrane.
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue sections using ACE2 Antibody (AGEL5342) at a dilution of 1:7500 (10× lens). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Western blot analysis of MCF-7,Mouse kindey,Mouse lung,Mouse heart and Rat heart cell lysates using ACE2 Antibody (AGEL5342) at a dilution of 1:1500. Observed-MW:120-135 kDa Calculated-MW:93 kDa
