The Recombinant B2M/beta-2 microglobulin Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110017) against B2M. This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of HeLa, HepG2, A431, Raji, Jurkat, Mouse spleen, Mouse liver, Rat spleen, Rat lung, Rat liver and IHC analysis of Human tonsil, Rat kidney. The target is localized to the secreted, cell surface.
B2M, B2MG, Beta 2 microglobin, Beta 2 microglobulin, Beta 2 microglobulin precursor, Beta chain of mhc class 1 proteins, Beta chain of MHC class I molecules, Beta-2-Microglobulin, Beta-2-microglobulin form pI, Beta-2-microglobulin form pI 5.3, CDABP, CDABP0092, HDCMA22P, IMD, IMD43, β2-Microglobulin, β2M
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human B2M / beta-2 microglobulin protein
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110017
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
B2M
Conjugation:
Unconjugated
Observed MW:
12 kDa
Calculated MW:
14 kDa
Recommended Dilution:
IHC 1:2000-10000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P61769, P01887, P07151
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
0.2 µm filtered solution in PBS
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue sections using B2M/beta-2 microglobulin Antibody (AGEL5336) at a dilution of 1:3000 (10× lens). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue sections using B2M/beta-2 microglobulin Antibody (AGEL5336) at a dilution of 1:3000 (10× lens). Heat mediated antigen retrieval was performed with Tris- EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Western blot analysis of HeLa,HepG2,A431,Raji,Jurkat,Mouse spleen,Mouse liver,Rat spleen,Rat lung and Rat liver cell lysates using B2M/beta-2 microglobulin Antibody (AGEL5336) at a dilution of 1:3000. Observed-MW:12kD Calculated-MW:14kD
