The Recombinant COUP-TFII Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C121601) against COUP-TFII. Chicken ovalbumin upstream promoter transcription factor (COUP-TF) belongs to the NR2 subfamily of the nuclear hormone receptor family. COUP-TFI and COUP-TFII are two of the well-characterized members in the NR2 subfamily. These two members are highly conserved in their two zinc-finger DNA binding domains (DBD) and the ligand binding domain (LBD), and function as repressors or activators of downstream target genes to regulate different biological processes. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC and ChIP applications. It has been validated by WB analysis of MCF-7, K562, PC-12, IHC analysis of Human testis, Human breast cancer, Mouse testis, Rat testis and ChIP analysis of MCF-7. The target is localized to the nucleus.
Product Name:
Recombinant COUP-TFII Monoclonal Antibody
SKU:
AGEL6906
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
Apolipoprotein A-I regulatory protein, Apolipoprotein A-I regulatory protein 1, Apolipoprotein AI regulatory protein, Apolipoprotein AI regulatory protein 1, ARP, ARP-1, ARP1, CHTD, CHTD4, COT, COT2, COUP TF, COUP TF2, COUP transcription factor, COUP transcription factor 2, COUP transcription factor II, COUP-TF, COUP-TF II, COUP-TF2, COUPTFB, COUPTFII, NF-E, NF-E3, NR2F1, NR2F2, Nuclear receptor subfamily 2 group F member, Nuclear receptor subfamily 2 group F member 2, SVP, SVP40, TFCOUP, TFCOUP2
Reactivity:
Human, Mouse, Rat
Immunogen:
Peptide. This information is proprietary to PTMab.
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Chromatin immunoprecipitation analysis of MCF-7 immunoprecipitated DNA by real-time PCR using primers specific for the human TACSTD2-E, CYP24A1-E1, CYP24A1-E2, ZNF217-E1 and ZNF217-E2. The data are presented as enrichment of each sample relative to the total amount of input chromatin at each amplicon.
Immunohistochemistry of paraffin-embedded Human testis using COUP-TFII Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Human breast cancer using COUP-TFII Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Mouse testis using COUP-TFII Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Rat testis using COUP-TFII Monoclonal Antibody at dilution of 1:100.
Western Blot with COUP-TFII Monoclonal Antibody at dilution of 1:1000. Lane 1: MCF-7, Lane 2: K562, Lane 3: PC-12 Observed-MW:46 kDa Calculated-MW:46 kDa
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