The Recombinant Cyclin B1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C371456) against Cyclin B1. Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB, IF and IP applications. It has been validated by WB analysis of Jurkat, HeLa, HT-29, IF analysis of Jurkat and IP analysis of HeLa cells extracts. The target is localized to the cytoplasm, cytoskeleton, nucleus.