The Recombinant EEA1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110693) against EEA1. Antibodies against EEA1 are found in sera from patients with subacute cutaneous lupus erythematosus and other autoimmune diseases., domain:The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate., Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking., similarity:Contains 1 C2H2-type zinc finger., similarity:Contains 1 FYVE-type zinc finger., subunit:Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of C6 and IHC analysis of Human breast ductal cancer tissue. The target is localized to the cytoplasm.
Product Name:
Recombinant EEA1 Monoclonal Antibody
SKU:
AGEL6002
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
162kD, Early endosome antigen 1, Early endosome associated protein, EEA, EEA 1, EEA1, Endosome associated protein p162, Endosome-associated protein p162, MST, MST105, MSTP, MSTP105, ZFYVE, ZFYVE2, Zinc finger FYVE domain containing protein 2, Zinc finger FYVE domain-containing protein 2
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human EEA1 protein
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110693
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
EEA1
Conjugation:
Unconjugated
Observed MW:
162 kDa
Calculated MW:
162 kDa
Recommended Dilution:
IHC 1:100-200; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
Q15075
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded human breast ductal cancer tissue using Recombinant EEA1 Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant EEA1 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: C6 cells. Observed-MW:162 kDa Calculated-MW:162 kDa