Thioredoxin; also known as ATL-derived factor; Surface-associated sulfhydryl protein; SASP and TXN; is a surface-associated nucleus, cytoplasm, and secreted thioredoxin family protein. Thioredoxins are proteins that function as antioxidants by helping to decrease other proteins through cysteine thiol-disulfide exchange. Thioredoxins are found in all known life forms, and they are required for mammal survival. Thioredoxin is involved in a variety of redox processes, including the reversible oxidation of its active center dithiol to a disulfide and dithiol-disulfide exchange reactions. The reversible S-nitrosylation of cysteine residues in target proteins is aided by thioredoxin; as a result, it contributes to the intracellular nitric oxide response. The active site Cys of CASP3 is nitrated by thioredoxin in response to nitric oxide (NO); as a result, caspase-3 activity is reduced. Thioredoxin activates the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells by changing the oxidation/reduction state and increasing transcriptional activity. Human Thioredoxin/TXN Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Thioredoxin/TXN Recombinant Protein (RPES2365)
Measured by its ability to catalyze the reduction of insulin. The specific activity is 5-9 pmoles/min/µg.2. Measured by its ability to catalyze the reduction of insulin. The reaction leads toprecipitation; which can be measured by absorbance at 650 nm. The specific activity is 5-10 A650/min/mg.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the C-terminal segment of human TXN (P10599) (Met 1-Val 105) was expressed and purified.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
11.7 kDa
AP Mol Mass:
14 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
