Human Thioredoxin-2/TXN2 Recombinant Protein (RPES2513)

Product type:
Recombinant Protein
Host species:


ELISA Kit Technical Manual

Recombinant Human Thioredoxin-2/TXN2 Protein (His Tag)(Active)

Background:Thioredoxin-2, also known as TXN2, MTRX and TRX2, is a member of the thioredoxin family. Tryparedoxins (TXN) are thioredoxin-related proteins which, as trypanothione:peroxiredoxin oxidoreductases, constitute the trypanothione-dependent antioxidant defense and may also serve as substrates for ribonucleotide reductase in trypanosomatids. Thioredoxin-2 / TXN2 contains one thioredoxin domain. It is widely expressed in adult (at protein level) and fetal tissues. Human Thioredoxin-2 / TXN2 is a small redox protein important in cellular antioxidant defenses, as well as in the regulation of apoptosis. Thioredoxin-2 / TXN2 has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Thioredoxin-2 / TXN2 could be involved in the resistance to anti-tumor agents. It possesses a dithiol-reducing activity. Thioredoxin-2 / TXN2 plays an important role in protecting the mitochondria against oxidative stress and in sensitizing the cells to ROS-induced apoptosis. Mammalian Thioredoxin-2 / TXN2 is a mitochondrial isoform of highly evolutionary conserved thioredoxins. Thioredoxins are small ubiquitous protein-disulfide oxidoreductases implicated in a large variety of biological functions.
Name:Recombinant Human Thioredoxin-2/TXN2 Protein (His Tag)(Active)
Assay Genie SKU:RPES2513
Synonyms:Thioredoxin Mitochondrial; MTRX; Mt-Trx; Thioredoxin-2; TXN2; TRX2
Expression host:E.coli
Sequence:Thr 60-Gly 166
Mol Mass:13.4 kDa
AP Mol Mass:13 kDa
Bio Activity:Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation, which can be measured by absorbance at 650 nm. The specific activity is 5-8 A650/min/mg.
Purity:> 97 % as determined by reducing SDS-PAGE.
Endotoxin:Please contact us for more information.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from sterile PBS, pH 8.3
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:TXN2: Has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Could be involved in the resistance to anti-tumor agents. Possesses a dithiol-reducing activity. Belongs to the thioredoxin family.
UniProt Protein Details:

Protein type:Oxidoreductase

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cell soma; mitochondrion; mitochondrial matrix; dendrite; nucleolus

Molecular Function:peptide-methionine-(S)-S-oxide reductase activity; protein complex binding; protein disulfide oxidoreductase activity; peptide-methionine (R)-S-oxide reductase activity

Biological Process: response to drug; cellular response to nutrient levels; response to reactive oxygen species; cell redox homeostasis; response to hormone stimulus; response to glucose stimulus; response to hypoxia; response to axon injury; response to organic cyclic substance; glycerol ether metabolic process; response to nutrient

NCBI Summary:This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. [provided by RefSeq, Jul 2008]
UniProt Code:Q99757
NCBI GenInfo Identifier:20455529
NCBI Gene ID:25828
NCBI Accession:Q99757.2
UniProt Secondary Accession:Q99757,Q5JZA0, Q6FH60, Q9UH29,
UniProt Related Accession:Q99757
Molecular Weight:
NCBI Full Name:Thioredoxin, mitochondrial
NCBI Synonym Full Names:thioredoxin 2
NCBI Official Symbol:TXN2
NCBI Official Synonym Symbols:MTRX; TRX2; MT-TRX
NCBI Protein Information:thioredoxin, mitochondrial; thioredoxin-2; mitochondrial thioredoxin
UniProt Protein Name:Thioredoxin, mitochondrial
UniProt Synonym Protein Names:Thioredoxin-2
UniProt Gene Name:TXN2
UniProt Entry Name:THIOM_HUMAN