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Key Features

Recombinant Prohibitin Monoclonal Antibody (AGEL8692)

Datasheet MSDS
SKU AGEL8692
Product Type Antibody
Reactivity Human, Rat
Applications WB, IHC-P
Host Species Rabbit
Clonality Monoclonal
Clone C723387
Conjugate Unconjugated
$461 - $632

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Description

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Recombinant Prohibitin Monoclonal Antibody

The Recombinant Prohibitin Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C723387) against Prohibitin. Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis. This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription. Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis. Produced by recombinant technology in Rabbit hosts and purified by affinity chromatography, this Rabbit IgG antibody is reactive against Human and Rat samples.

This antibody is suitable for WB and IHC-P applications. It has been validated by WB analysis of Hela, A549, HL-60, U2OS, C6 and IHC analysis of Human Cholangiocarcinoma. The target is localized to the mitochondrion inner membrane.

Product Name:Recombinant Prohibitin Monoclonal Antibody
SKU:AGEL8692
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:Epididymis luminal protein 215, Epididymis secretory sperm binding protein Li 54e, HEL 215, HEL S 54e, PHB, PHB1, Prohibitin
Reactivity:Human, Rat
Immunogen:A synthetic peptide of human Prohibitin
Applications:WBIHC-P
Clonality:Recombinant Monoclonal
Clone No:C723387
Host:Rabbit
Isotype:Rabbit IgG
Target:Prohibitin
Conjugation:Unconjugated
Observed MW:30 kDa
Calculated MW:30 kDa
Recommended Dilution:WB 1:500-1000; IHC 1:50-100
UniProt:P35232
Purification:Affinity purification
Concentration:0.3 mg/mL
Buffer:50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
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