The Recombinant RhoA Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110737) against RhoA. This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of NIH3T3 and IHC analysis of Human placenta tissue. The target is localized to the Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex, Midbody. Cell projection, lamellipodium, cell projection, dendrite, nucleus, Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in an activated form and in a myosin- and actin-independent manner.
Product Name:
Recombinant RhoA Monoclonal Antibody
SKU:
AGEL6045
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
Aplysia ras related homolog 12, ARH, ARH12, ARHA, EDFAOB, H 12, H12, Oncogene RHO H12, ras homolog family member A, Ras homolog gene family member A, Rho, Rho A, Rho cDNA clone 12, RHO H12, RHO12, RHOA, RHOH, RHOH12, Small GTP binding protein Rho A, Transforming protein Rho A, Transforming protein RhoA
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human RhoA protein
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110737
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
RhoA
Conjugation:
Unconjugated
Observed MW:
21 kDa
Calculated MW:
21 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P61586
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
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