Description
RFX3 Transcription Factor Activity Assay
The RFX3 Transcription Factor Activity Assay Kit is a comprehensive and reliable tool for studying the transcriptional activity of the RFX3 transcription factor. This kit is designed for accurate detection and quantification of RFX3 activity in cell lysates, serum, and tissue samples. With high sensitivity and specificity, researchers can trust the results obtained from this kit for a wide range of research applications.The RFX3 transcription factor plays a crucial role in regulating gene expression and cell cycle progression, making it a key player in various biological processes and diseases.
By studying the activity of RFX3, researchers can gain valuable insights into the mechanisms underlying cancer, immune response, and developmental disorders. With the RFX3 Transcription Factor Activity Assay Kit, researchers can advance their understanding of RFX3 function and potentially identify new therapeutic targets for various diseases.
| Product Name: | RFX3 Transcription Factor Activity Assay |
| Product Code: | TFAB00157 |
| Target: | RFX3 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie RFX3 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | RFX3: Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left- right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Acts as a transcription factor. Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells. Belongs to the RFX family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; DNA-binding Chromosomal Location of Human Ortholog: 9p24.2 Cellular Component: nuclear chromatin; nucleus; transcription factor complex Molecular Function:DNA binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding Biological Process: cell maturation; cilium biogenesis; endocrine pancreas development; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of insulin secretion; regulation of transcription, DNA-dependent |
| NCBI Summary: | This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013] |
| UniProt Code: | P48380 |
| NCBI GenInfo Identifier: | 32172437 |
| NCBI Gene ID: | 5991 |
| NCBI Accession: | P48380.2 |
| UniProt Secondary Accession: | P48380,Q5JTL7, Q5JTL8, Q6NW13, Q8WTU4, Q95HL5, Q95HL6 A8K0H5, D3DRH8, D3DRH9, |
| UniProt Related Accession: | P48380 |
| Molecular Weight: | 45,715 Da |
| NCBI Full Name: | Transcription factor RFX3 |
| NCBI Synonym Full Names: | regulatory factor X3 |
| NCBI Official Symbol: | RFX3 |
| NCBI Protein Information: | transcription factor RFX3 |
| UniProt Protein Name: | Transcription factor RFX3 |
| UniProt Synonym Protein Names: | Regulatory factor X 3 |
| Protein Family: | Transcription factor |
| UniProt Gene Name: | RFX3 |
| UniProt Entry Name: | RFX3_HUMAN |
