Anti-RPL30 Antibody (CAB13690)

Antibody Name:	Anti-RPL30 Antibody
Antibody SKU:	CAB13690
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human RPL30 (NP_000980.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, MCF7, HepG2, A-549, Mouse brain, Mouse heart, Mouse kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human RPL30 (NP_000980.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVAA KKTK KSLE SINS RLQL VMKS GKYV LGYK QTLK MIRQ GKAK LVIL ANNC PALR KSEI EYYA MLAK TGVH HYSG NNIE LGTA CGKY YRVC TLAI IDPG DSDI IRSM PEQT GEK
Gene ID:	6156
Uniprot:	P62888
Cellular Location:
Calculated MW:	12kDa
Observed MW:	13kDa

Synonyms:

RPL30, L30

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPL30: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 8q22.2 Cellular Component: cytosol; extracellular matrix; focal adhesion; membrane; nucleus Molecular Function:protein binding; RNA binding; structural constituent of ribosome Biological Process: defense response to Gram-negative bacterium; killing of cells of another organism; mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P62888
NCBI GenInfo Identifier:	4506631
NCBI Gene ID:	6156
NCBI Accession:	NP_000980.1
UniProt Secondary Accession:	P62888,P04645, Q502Z6, B2R591,
UniProt Related Accession:	P62888
Molecular Weight:	40.2kD
NCBI Full Name:	60S ribosomal protein L30
NCBI Synonym Full Names:	ribosomal protein L30
NCBI Official Symbol:	RPL30
NCBI Official Synonym Symbols:	L30
NCBI Protein Information:	60S ribosomal protein L30
UniProt Protein Name:	60S ribosomal protein L30
UniProt Synonym Protein Names:	Large ribosomal subunit protein eL30
Protein Family:	60S ribosomal protein
UniProt Gene Name:	RPL30

	Western blot analysis of extracts of various cell lines, using RPL30 antibody (CAB13690) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat heart using RPL30 antibody (CAB13690) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using RPL30 antibody (CAB13690) at dilution of 1:100 (40x lens).
	Confocal immunofluorescence analysis of HeLa cells using RPL30 antibody (CAB13690) at dilution of 1:200. Blue: DAPI for nuclear staining.

Antibodies

RPL30 Antibody (PACO01442)

RPL30 Antibody (PACO11923)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)