The RPS20 Monoclonal Antibody (CAB9551) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for a variety of research applications, particularly in Western blotting.RPS20 is a key component of the ribosome, the cellular machinery responsible for protein production. Dysregulation of RPS20 has been linked to various diseases, including cancer and genetic disorders. By targeting RPS20 with this monoclonal antibody, researchers can investigate its expression levels and localization in different cell types, shedding light on its function in normal and diseased states.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RPS20 Monoclonal Antibody
SKU:
CAB9551
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1630
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
S20, uS10, RPS20
Positive Sample:
HeLa, Hep G2, Jurkat, Mouse testis, Mouse spleen, Rat lung, Rat testis, Rat spleen
Cellular Localization:
Cytoplasm.
Calculated MW:
13kDa
Observed MW:
16kDa
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene.
Purification Method
Affinity purification
Gene ID
6224
RRID
AB_2863721
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RPS20 Rabbit mAb (CAB9551) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using RPS20 Rabbit mAb (CAB9551) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using RPS20 Rabbit mAb (CAB9551) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using RPS20 Rabbit mAb (CAB9551) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of C6 cells using RPS20 Rabbit mAb (CAB9551) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation of RPS20 in 300 µg extracts from Hep G2 cells using 3 µg RPS20 Rabbit mAb (CAB9551). Western blot analysis was performed using RPS20 Rabbit mAb (CAB9551) at 1:1000 dilution.
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