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    Understanding the mTOR Signaling Pathway: A Key Player in Cell Growth and Metabolism(Post)

    Cell Signalling · MetabolismThe mTOR Signalling PathwaymTOR is a master regulator of cell growth, metabolism and survival, integrating nutrient, growth-factor and energy signals through two comp

    In the Thymus, Co-stimulation Kills(Post)

    In the periphery, co-stimulation rescues a T cell. In the thymus, the same molecules kill it. A peripheral T cell that engages its receptor without CD80 and CD86 becomes anergic; engage them and it r

    No Cytokine on This Pathway Touches Bone(Post)

    Not one immune cytokine on this pathway touches bone. TNF, IL-1β, IL-6 and IL-17A all act on osteoblasts, osteocytes and synovial fibroblasts, and it is those stromal cells that decide between RANKL

    Ferroptosis Assays(Page)

    Ferroptosis Assay Kits | Lipid Peroxidation, Iron & Glutathione | Assay Genie

    Assay Genie launch SARS-CoV-2 qPCR assay(Post)

    SARS-CoV-2 qPCR assay from Assay Genie Assay Genie have just launched a qPCR assay for the detection of SARS-CoV-2. The SARS-CoV-2 One-Step RT-qPCR Kit for Coro

    Mycoplasma Testing - Detection & Elimination(Page)

    Dendritic Cells - Markers, Activation & Subtypes(Post)

    Immunology · Antigen PresentationDendritic Cells: Markers, Activation and SubtypesDendritic cells are the professional antigen-presenting cells that decide whether an immune response happens at all. T

    3D Cell Culture: Assay Guide(Post)

    References Key Points Three-dimensional (3D) cell cultures are artificially-created environments in which cells are permitted to grow or interact with their surroundings in a 3D fa

    Annexin V Assays(Page)

    Annexin V Apoptosis Assay Kits | Flow Cytometry Detection | Assay Genie

    #LabChats with Laura Menke(Post)

    At ELISA Genie we are hugely interested in the future of science and we feel that PhD students are at the heart of this. Our new series, #LabChats, will see us interview different scienti

    T cell Immunophenotyping - A Guide(Post)

    Immunology · Flow CytometryT Cell Immunophenotyping: Markers, Subsets and Panel DesignImmunophenotyping identifies cell populations by the proteins on their surface. For T cells that means resolving n

    Evita falsos positivos: isotipos y controles para investigadores(Post)

    Un isotipo es la clase de inmunoglobulina que define la región constante de su cadena pesada, y el organismo humano produce cinco: IgG, IgA, IgM, IgD e IgE. Cada uno tiene una forma distinta, apar

    Evite 3–6 meses de retrabajo: selección de anticuerpos para investigadores(Post)

    Evite 3–6 meses de retrabajo: selección de anticuerpos para investigadores La selección de anticuerpos consiste en identificar y validar la molécula con la especificidad y el desempeño necesarios par

    One Ligand, Two Receptors, Opposite Jobs: TNFR1 vs TNFR2(Post)

    Blocking TNF cures rheumatoid arthritis in some patients and triggers demyelination in others. That is not a dosing problem, and it is not a patient-selection problem. It is the direct consequence of

    Binding Is the Easy Part(Post)

    An antibody–drug conjugate is not a targeted drug. It is a delivery problem with an antibody bolted to the front of it. Binding is the easy part, and it is almost never the part that fails. What deci

    The Role of ACE2 in SARS-CoV-2(Post)

    Structure, Function, and Detection Assays for COVID-2019 Background The global spread of the novel cor

    Your Th2 Phenotype Might Not Be a T Cell(Post)

    If your knockout showed a type 2 phenotype and you attributed it to Th2 cells, there is a reasonable chance you were looking at something else. Innate lymphoid cells run T-bet, GATA3 and RORγt — not

    CD4 T-Helper Differentiation: Th1, Th2, Th17, Treg and Tfh(Post)

    The antigen tells a CD4 T cell to respond; the cytokine tells it what kind of response to make. A naive CD4⁺ T cell leaving the thymus is uncommitted. Within about seventy-two hours of engaging TCR/C

    Oxidative Stress Assays(Page)

    Oxidative Stress Assay Kits | ROS, Antioxidant & Glutathione | Assay Genie

    PhD Summit - Two Days of Inspiring Talks(Post)

    PhD Summit - A Virtual Conference ELISA Genie is proud to announce the launch of a groundbreaking virtual conference - PhD Summ