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Sandwich ELISA: Step-by-Step Protocol & Troubleshooting(Post)
Written by Seán Mac Fhearraigh, PhD • Updated: 19 May 2026 • ~10 min read Quick Answer A sandwich ELISA uses two antibodies — a capture ant
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Featured
Human CAT (Catalase) ELISA Kit (HUFI07676)
- Product Code:
- HUFI07676
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 9.375 pg/mL
- Range:
- 15.625-1000 pg/mL
Human MICA ELISA Kit (HUFI00015)
- Product Code:
- HUFI00015
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 46.875 pg/mL
- Range:
- 78.125-5000 pg/mL
Fatty Acid Oxidation (FAO) Assay Kit (BR00001)
- Product Code:
- BR00001
- Instrument:
- Microplate Reader
Anti-Mouse IFNAR-1 In Vivo Antibody - Low Endotoxin (IVMB0202)
- Product Code:
- IVMB0202
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- MAR1-5A3
- Protein:
- IFNAR1
- Isotype:
- Mouse IgG1
Anti-Mouse CD25 In Vivo Antibody - Low Endotoxin (IVMB0148)
- Product Code:
- IVMB0148
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- PC61
- Protein:
- CD25
- Isotype:
- Rat IgG1 lambda
Anti-Mouse NK1.1 In Vivo Antibody - Low Endotoxin (IVMB0100)
- Product Code:
- IVMB0100
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- PK136
- Protein:
- NK1.1
- Isotype:
- Mouse IgG2a kappa
![Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214372/569320/anti-mouse-pd-1-cd279-rmp1-14-in-vivo-antibody-low-endotoxin__18202.1743527968.jpg?c=2 "Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody - Low Endotoxin")
Anti-Mouse PD-1 Antibody [RMP1-14] In Vivo-Low Endotoxin (IVMB0037)
- Product Code:
- IVMB0037
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- RMP1-14
- Protein:
- PD-1
- Isotype:
- Rat IgG2a kappa
LSAMP Rabbit Polyclonal Antibody (CAB14248)
- Product Code:
- CAB14248
- Antibody Type:
- Polyclonal Antibody
MCM4 Rabbit Polyclonal Antibody (CAB13513)
- Product Code:
- CAB13513
- Antibody Type:
- Polyclonal Antibody
VEGF Rabbit Polyclonal Antibody (CAB12303)
- Product Code:
- CAB12303
- Antibody Type:
- Polyclonal Antibody
MDA ELISA Kit (UNFI0048)
- Product Code:
- UNFI0048
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 4.688 ng/mL
- Range:
- 7.813-500 ng/mL
Dopamine ELISA Kit (UNFI0013)
- Product Code:
- UNFI0013
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 0.938 ng/mL
- Range:
- 1.563-100 ng/mL
Serotonin ELISA Kit (UNFI0018)
- Product Code:
- UNFI0018
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 0.938 ng/mL
- Range:
- 1.563-100 ng/mL
PGE2 ELISA Kit (UNFI0032)
- Product Code:
- UNFI0032
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 18.75 pg/mL
- Range:
- 31.25-2000 pg/mL
Human IL-6 ELISA Kit (HUFI00180)
- Product Code:
- HUFI00180
- Sensitivity:
- 2.813pg/ml
- Range:
- 4.688-300pg/ml
- ELISA Type:
- Sandwich
