The SEMA3C Antibody (CAB15386) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for SEMA3C and has been validated for use in Western blot and immunohistochemistry applications. It binds to the SEMA3C protein, allowing for accurate detection and analysis in a variety of cell types.SEMA3C is known for its involvement in processes such as cell migration, angiogenesis, and neuronal development, making it a key player in various physiological and pathological conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SEMA3C Antibody
SKU:
CAB15386
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SemE, SEMAE, SEMA3C
Positive Sample:
Human serum
Cellular Localization:
Secreted.
Calculated MW:
85kDa
Observed MW:
85kDa
This gene encodes a secreted glycoprotein that belongs to the semaphorin class 3 family of neuronal guidance cues. The encoded protein contains an N-terminal sema domain, integrin and immunoglobulin-like domains, and a C-terminal basic domain. Homodimerization and proteolytic cleavage of the C-terminal propeptide are necessary for the function of the encoded protein. It binds a neuropilin co-receptor before forming a heterotrimeric complex with an associated plexin. An increase in the expression of this gene correlates with an increase in cancer cell invasion and adhesion. Naturally occurring mutations in this gene are associated with Hirschsprung disease.
Purification Method
Affinity purification
Gene ID
10512
RRID
AB_2762292
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Human serum, using SEMA3C Rabbit pAb (CAB15386) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of L929 cells using SEMA3C Rabbit pAb (CAB15386) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
