The [KO Validated] SQSTM1/p62 Antibody (CAB11483) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the Sequestosome-1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential asset for studies in cell biology and neurodegenerative diseases.Sequestosome-1, also known as p62/SQSTM1, is a multifunctional protein involved in various cellular processes, including selective autophagy and signaling pathways related to inflammation and oxidative stress. Its role in protein degradation and quality control makes it a key player in maintaining cellular homeostasis and preventing the accumulation of damaged proteins.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] SQSTM1/p62 Antibody
SKU:
CAB11483
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
Purification Method
Affinity purification
Gene ID
8878
RRID
AB_2861579
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SQSTM1/p62 Rabbit pAb (CAB11483) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of U2OS cells using SQSTM1/p62 Rabbit pAb (CAB11483) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100 μg extracts of HepG2 cells using 3 μg SQSTM1/p62 antibody (CAB11483). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (CAB11483) at a dilution of 1:1000.
