Description
Sorbitol Dehydrogenase (SORD) Activity Assay Kit
The Assay Genie Sorbitol Dehydrogenase (SORD) Activity Assay Kit measures SORD (L-iditol 2-dehydrogenase, EC 1.1.1.14) activity in cell and tissue lysates. SORD oxidises sorbitol to fructose using NAD+ and, together with aldose reductase, forms the polyol pathway. Increased flux through this pathway under hyperglycaemia is implicated in diabetic complications, and SORD deficiency has been linked to inherited peripheral neuropathy.
NADH generated by SORD reduces the tetrazolium dye INT to a cherry-red formazan read at 492 nm. Incubation times of just 30 or 60 minutes and direct calculation in IU/L make this a quick and convenient polyol pathway assay.
| Product Name: | Sorbitol Dehydrogenase Assay Kit |
| SKU: | BR00011 |
| Kit Size: | 100 wells |
| Detection Method: | Colorimetric (INT → formazan, OD 492 nm) |
| Sample Types: | Cell and tissue lysates |
| Recommended Protein: | 0.2–3 mg/ml |
| Incubation Time: | 30 or 60 min at 37 °C |
| Units: | IU/L (µmol/L/min); normalise to protein or GAPDH |
| Equipment Required: | Microplate reader (492 nm) |
| Storage: | -80 °C (see component storage) |
| Intended Use: | For research use only (RUO). Not for diagnostic procedures. |
- Short incubation: results in 30–60 min
- Direct activity calculation in IU/L, no standard curve required
- Per-sample no-substrate control
- Simple 96-well mix-and-read format
- Includes 10x Sample Buffer for lysate preparation
- SORD oxidises sorbitol to fructose, reducing NAD+ to NADH.
- NADH drives the reduction of INT to a coloured formazan. Formazan has an absorbance maximum at 492 nm (ε = 18 mM-1cm-1), so activity is calculated directly in IU/L (µmol/L/min) without a standard curve.
- Activity is calculated from ΔOD (reaction − control): IU/L = ΔOD × 16.2 (30 min) or × 8.1 (60 min).
- Cells: ≥105 PBS-washed, frozen cells in 50–100 µl ice-cold 1x Sample Buffer.
- Tissue: ~5 mg tissue homogenised in 500 µl ice-cold 1x Sample Buffer.
- 5 min on ice, then centrifuge at ~14,000 rpm for 5 min; adjust protein to 0.2–3 mg/ml.
| Component | Size | Storage |
| SORD Assay Solution | 5 ml | -80 °C, protect from light |
| 10x SORD Substrate | 0.5 ml | -80 °C |
| 10x Sample Buffer | 25 ml | 4 °C |
- Polyol pathway and fructose metabolism
- Diabetic complications (neuropathy, retinopathy, nephropathy)
- Inherited SORD-deficiency neuropathy models
- Liver and lens metabolism studies
How long does the SORD assay take?
The incubation is 30 or 60 min at 37 °C, plus a short lysate preparation.
How is SORD activity calculated?
ΔOD at 492 nm (reaction − control) × 16.2 for 30 min or × 8.1 for 60 min, giving IU/L.
What if activity is too low?
Increase the sample protein concentration (up to 3 mg/ml) or use the longer incubation.
