Sorbitol Dehydrogenase Assay Kit (BA0056)
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Sorbitol Dehydrogenase Assay Kit - InformationAssay Genie's non-radioactive, colorimetric SDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
ApplicationsFor quantitative determination of sorbitol dehydrogenase enzyme activity.
Sorbitol Dehydrogenase Assay Kit - Key Features
- Fast and sensitive. Linear detection range (20 sample): 0.1 to 125 U/L for 12 min reaction.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Sorbitol Dehydrogenase Assay Kit - Data Sheet
|Kit Includes||Assay Buffer: 10 mL Diaphorase: 120 uL Substrate: 250 uL Calibrator: 1.5 mL NAD/MTT Solution: 1 mL|
|Kit requires||Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat-bottom 96-well plates, centrifuge tubes and plate reader|
|Method of Detection||OD565nm|
|Detection Limit||0.1 U/L|
|Samples||Biological samples e.g. plasma, serum, urine, tissue and culture media.|
|Protocol Length||15 min|
|Shelf Life||6 months|
|Storage||Store all components at -20°C upon receiving|
Aliases for SORD Gene
- L-Iditol 2-Dehydrogenase
- EC 184.108.40.206
- Epididymis Secretory Sperm Binding Protein Li 95n
- EC 1.1.1
Entrez Gene Summary for SORD GeneSorbitoldehydrogenase(SORD; EC 220.127.116.11) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up thesorbitolpathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose tosorbitolby ALDR1 with NADPH as the cofactor. SORD then oxidizes thesorbitolto fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]
UniProtKB/Swiss-Prot for SORD GeneDHSO_HUMAN,Q00796Convertssorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm.
Protein details for SORD Gene (UniProtKB/Swiss-Prot)Protein Symbol:Q00796-DHSO_HUMANRecommended name:SorbitoldehydrogenaseProtein Accession:Q00796Secondary Accessions:B2R655, B7Z3A6, J3JZZ5, Q16682, Q9UMD6
Protein attributes for SORD GeneSize:357 amino acidsMolecular mass:38325 DaCofactor:Name=Zn(2+); Xref=ChEBI:CHEBI:29105;Quaternary structure:Homotetramer.Miscellaneous:The polyol pathway is proposed to be involved the cellular toxicity of diabetic hyperglycemia.
Post-translational modifications for SORD Gene
- Ubiquitination at Lys 107 and Lys 134
- Modification sites at PhosphoSitePlus