Western blot analysis of extracts of various cell lines, using Sorbitol Dehydrogenase antibody (CAB21952) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Sorbitol Dehydrogenase Monoclonal Antibody (CAB21952) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody is specifically designed for use in research related to sorbitol dehydrogenase, a crucial enzyme in the sorbitol pathway.Raised in mice, this antibody is highly specific and sensitive, making it an ideal choice for detecting and analyzing sorbitol dehydrogenase in various biological samples. It has been validated for use in Western blot applications, providing reliable and reproducible results.Sorbitol dehydrogenase is involved in the conversion of sorbitol to fructose, a process that is essential for normal metabolic function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Sorbitol Dehydrogenase Monoclonal Antibody
SKU:
CAB21952
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC53945
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
REND EFCK MGRY NLSP SIFF CATP PDDG NLCR FYKH NAAF CYKL PDNV TFEE GALI EPLS VGIH ACRR GGVT LGHK VLVC GAGP IGMV TLLV AKAM GAAQ V
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:20000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.
Purification Method
Affinity purification
Gene ID
6652
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Sorbitol Dehydrogenase Rabbit mAb (CAB21952) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from HT-29 cells, using Sorbitol Dehydrogenase Rabbit mAb (CAB21952) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
