Anti-TMPRSS2 Antibody (CAB1979)

Antibody Name:	Anti-TMPRSS2 Antibody
Antibody SKU:	CAB1979
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 140-400 of human TMPRSS2 (NP_001128571.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HT-29, PC-3, Mouse kidney, Mouse stomach, Rat large intestine, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 140-400 of human TMPRSS2 (NP_001128571.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GLLW KFMG SKCS NSGI ECDS SGTC INPS NWCD GVSH CPGG EDEN RCVR LYGP NFIL QVYS SQRK SWHP VCQD DWNE NYGR AACR DMGY KNNF YSSQ GIVD DSGS TSFM KLNT SAGN VDIY KKLY HSDA CSSK AVVS LRCI ACGV NLNS SRQS RIVG GESA LPGA WPWQ VSLH VQNV HVCG GSII TPEW IVTA AHCV EKPL NNPW HWTA FAGI LRQS FMFY GAGY QVEK VISH PNYD SKTK NNDI ALMK LQKP LTFN DLVK P
Gene ID:	7113
Uniprot:	O15393
Cellular Location:	Cell membrane, Secreted, Single-pass type II membrane protein
Calculated MW:	53kDa/57kDa
Observed MW:	70KDa/54KDa/32KDa

Synonyms:

TMPRSS2, PP9284, PRSS10

Background:

This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	TMPRSS2: a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
UniProt Protein Details:	Protein type:EC 3.4.21.-; Protease; Membrane protein, integral Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:protein binding; serine-type peptidase activity Biological Process: positive regulation of virion penetration into host cell; protein autoprocessing; proteolysis
NCBI Summary:	This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
UniProt Code:	O15393
NCBI GenInfo Identifier:	115502469
NCBI Gene ID:	7113
NCBI Accession:	O15393.3
UniProt Secondary Accession:	O15393,Q6GTK7, Q9BXX1, A8K6Z8, B2R8E5, B7Z459, D3DSJ2 F8WES1,
UniProt Related Accession:	O15393
Molecular Weight:	57,690 Da
NCBI Full Name:	Transmembrane protease serine 2
NCBI Synonym Full Names:	transmembrane protease, serine 2
NCBI Official Symbol:	TMPRSS2
NCBI Official Synonym Symbols:	PP9284; PRSS10
NCBI Protein Information:	transmembrane protease serine 2
UniProt Protein Name:	Transmembrane protease serine 2
UniProt Synonym Protein Names:	Serine protease 10
Protein Family:	Transmembrane protease serine
UniProt Gene Name:	TMPRSS2
UniProt Entry Name:	TMPS2_HUMAN

	Western blot analysis of extracts of various cell lines, using TMPRSS2 antibody (CAB1979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts of various cell lines, using TMPRSS2 antibody (CAB1979) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using TMPRSS2 Rabbit pAb (CAB1979) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded human placenta using TMPRSS2 Rabbit pAb (CAB1979) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using TMPRSS2 Rabbit pAb (CAB1979) at dilution of 1:50 (40x lens).

Antibodies	ELISA Kits
Anti-TMPRSS2 Antibody (CAB9126)	Human TMPRSS2 / Transmembrane Protease, Serine 2 ELISA Kit
	Human TMPRSS2 (Transmembrane Protease, Serine 2) CLIA Kit (HUES00807)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)