The TRIM25 Antibody (CAB12938) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high specificity and sensitivity towards human samples, making it an ideal choice for Western blotting applications.TRIM25, also known as tripartite motif-containing protein 25, plays a crucial role in the innate immune response by functioning as an E3 ubiquitin ligase and regulating the activation of the RIG-I signaling pathway. By targeting viral RNA and promoting its degradation, TRIM25 contributes to the antiviral defense mechanisms within the host.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TRIM25 Antibody
SKU:
CAB12938
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EFP, Z147, RNF147, ZNF147, TRIM25
Positive Sample:
HeLa, 293T, MCF7
Cellular Localization:
Cytoplasm.
Calculated MW:
71kDa
Observed MW:
72kDa
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein is an RNA binding protein, functions as a ubiquitin E3 ligase and is involved in multiple cellular processes, including regulation of antiviral innate immunity.
Purification Method
Affinity purification
Gene ID
7706
RRID
AB_2736877
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using TRIM25 Rabbit pAb (CAB12938) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded Human kidney using TRIM25 Rabbit pAb (CAB12938) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using TRIM25 Rabbit pAb (CAB12938) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of A-431 cells using TRIM25 Rabbit pAb (CAB12938) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of HepG2 cells, using 3 μg TRIM25 antibody (CAB12938). Western blot was performed from the immunoprecipitate using TRIM25 antibody (CAB12938) at a dilution of 1:1000.
