The [KO Validated] Cleaved PARP1 p25 Monoclonal Antibody (CAB19612) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody specifically targets the cleaved PARP (poly(ADP-ribose) polymerase) p25 protein, a well-known marker of apoptosis, making it an essential reagent for investigating cell death pathways.Raised in rabbits, this polyclonal antibody exhibits high specificity and sensitivity in detecting cleaved PARP p25 in human samples. It has been rigorously validated for use in Western blot applications, providing reliable results for researchers studying apoptosis in various cell types. The cleaved PARP p25 protein is a key player in the signaling cascade of apoptosis, serving as a substrate for caspases and indicating the activation of programmed cell death processes.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Jurkat treated with Etoposide, NIH/3T3 treated with Staurosporine, HeLa treated with Staurosporine
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
113kDa
Observed MW:
25kDa
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Purification Method
Affinity purification
Gene ID
142
RRID
AB_2862700
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and Cleaved PARP1 p25 knockout (KO) cells using Cleaved PARP1 p25 Rabbit mAb (CAB19612) at 1:10000 dilution incubated at room temperature for 1.5 hours. Wild type (WT) and PARP1 knockout (KO) HeLa cells were treated with Staurosporine(1 μM) at 37℃ for 6 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from Jurkat cells using Cleaved PARP1 p25 Rabbit mAb (CAB19612) at 1:10000 dilution incubated at room temperature for 1.5 hours. Jurkat cells were treated with Etoposide (25 μM) at 37℃ for 5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunoprecipitation of Cleaved PARP1 p25 from 600 µg extracts of Jurkat cells was performed using 2 µg of Cleaved PARP1 p25 Rabbit mAb (CAB19612). Rabbit IgG isotype control (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Cleaved PARP1 p25 Rabbit mAb (CAB19612) at a dilution of 1:1000.
