The FHL2 Monoclonal Antibody (CAB3670) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified. RRID AB_2863109 Gene ID 2274 Swiss Prot Synonym DRAL; AAG11; FHL-2; SLIM3; SLIM-3; FHL2
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FHL2 Monoclonal Antibody
SKU:
CAB3670
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2068
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DRAL, AAG11, FHL-2, SLIM3, SLIM-3, FHL2
Positive Sample:
Mouse heart, Rat heart, SKOV3, RD
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
32kDa
Observed MW:
32kDa
This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified. RRID AB_2863109 Gene ID 2274 Swiss Prot Synonym DRAL; AAG11; FHL-2; SLIM3; SLIM-3; FHL2
Purification Method:
Affinity purification
Gene ID:
2274
RRID:
AB_2863109
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FHL2 Rabbit mAb (CAB3670) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates using FHL2 Rabbit mAb (CAB3670) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
