Canine Interleukin Array (11 targets) (SARB0009)
- SKU:
- SARB0009
- Product Type:
- Protein Array
- Analytes:
- IL-1 alpha (IL-1 F1)
- IL-1 beta (IL-1 F2)
- IL-1 Ra (IL-1 F3)
- IL-10
- IL-12 p40
- IL-13
- IL-17A
- IL-2
- IL-21
- IL-6
- IL-8 (CXCL8)
- Reactivity:
- Canine
- Applications:
- Multiplex Array
Frequently bought together:
Description
system_update_altDatasheet
Product Sku: | SARB0009 |
Size: | 2, 4, or 8 |
Species Detected: | Canine |
Number of Targets Detected: | 11 |
Gene Symbols: | CXCL8, IL10, IL12B, IL17A, IL1B, IL2, IL6 |
Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
Design Principle: | Sandwich-based |
Method of Detection: | Chemiluminescence |
Pathway: | |
Quantitative/Semi-Quantitative: | Semi-Quantitative |
Solid Support: | Membrane |
Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
Hover over target for more information | ||||
---|---|---|---|---|
IL-1 alpha (IL-1 F1) | IL-1 beta (IL-1 F2)
| IL-1 Ra (IL-1 F3) | IL-10
| IL-12 p40
|
IL-13 | IL-17A
| IL-2
| IL-21 | IL-6
|
IL-8 (CXCL8)
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Canine Interleukin Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1. | Block membranes |
2. | Incubate with Sample |
3. | Incubate with Biotinylated Detection Antibody Cocktail |
4. | Incubate with HRP-Conjugated Streptavidin |
5. | Incubate with Detection Buffers |
6. | Image with chemiluminescent imaging system |
7. | Perform densitometry and analysis |