Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-Lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.
Assay Genie's Fluorometric D-lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at Ex/Em = 530/585 nm, is proportional to the lactate concentration in the sample.Description
D-Lactate Assay Kit
Applications
For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.
Key Features
- Sensitive and accurate. Detection limit of 1 uM and linearity up to 50 uM D-lactate in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
D-Lactate Assay Kit Information:
| Key Information | Description |
| Kit Includes: |
|
| Kit Requires: | Pipetting (multi-channel) devices. Black, flat bottom 96-well plates and fluorescent plate reader capable of reading at lex/em = 530/585 nm. |
| Method of Detection: | FL530/585 nm |
| Detection Limit: | 1 uM |
| Samples: | Serum, plasma, cell culture media etc |
| Species: | All |
| Protocol Length: | 60 min |
| Size: | 100 tests |
| Storage: | Store all reagents at -20°C |
| Shelf Life: | 6 months |
