L-Lactate Assay Kit (Fluorometric) (BA0104)

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SKU:
BA0104
€667

Description

ELISA Kit Technical ManualMSDS

L-lactate assay kit

LACTATE is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.

Assay Genie's Fluorometric L-lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at Ex/Em = 530/585 nm, is proportional to the lactate concentration in the sample.

Applications

For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.

Key Features

  • Sensitive and accurate. Detection limit of 1 uM and linearity up to 50 uM L-lactate in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay.
  • High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

L-lactate assay kit Information:

Kit Includes: Assay Buffer: 10 mL Enzyme A: 120 mL NAD Solution: 1 mL Enzyme B: 120 mL Probe: 750 mL Standard: 1 mL
Kit Requires: Pipetting (multi-channel) devices. Black, flat bottom 96-well plates and fluorescent plate reader capable of reading at FL »ex/em = 530/585 nm.
Method of Detection: FL530/585 nm
Detection Limit: 1 uM
Samples: Serum, plasma, cell culture media etc
Species: All
Protocol Length: 60 min
Size: 100 tests
Storage: Store all reagents at -20°C
Shelf Life: 6 months

 

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Additional Information

Product type:
Assay
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