D-Lactate Assay Kit (Fluorometric) (BA0100)

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SKU:
BA0100
€649

Description

ELISA Kit Technical ManualMSDS

D-Lactate Assay Kit

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-Lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.

Assay Genie's Fluorometric D-lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at Ex/Em = 530/585 nm, is proportional to the lactate concentration in the sample.

Applications

For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

Key Features

  • Sensitive and accurate. Detection limit of 1 uM and linearity up to 50 uM D-lactate in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay.
  • High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

D-Lactate Assay Kit Information:

Key Information Description
Kit Includes:
  • Assay Buffer: 10 mL
  • Enzyme A: 120 mL
  • NAD Solution: 1 mL
  • Enzyme B: 120 mL
  • Probe: 750 mL
  • Standard: 1 mL
Kit Requires:
Pipetting (multi-channel) devices. Black, flat bottom 96-well plates and fluorescent plate reader capable of reading at lex/em = 530/585 nm.
Method of Detection:
FL530/585 nm
Detection Limit:
1 uM
Samples:
Serum, plasma, cell culture media etc
Species:
All
Protocol Length:
60 min
Size:
100 tests
Storage:
Store all reagents at -20°C
Shelf Life:
6 months
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Additional Information

Product type:
Assay
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