L- Lactate Assay Kit (Colorimetric) (BA0091)

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SKU:
BA0091

Description

L- Lactate Assay Kit - Information

Assay Genie's colorimetric L- lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

Applications

For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.

L- Lactate Assay Kit- Key Features

  • Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed.
  • High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

L- Lactate Assay Kit - Data Sheet

Method of DetectionOD565nm
Detection Limit0.05 mM
SamplesSerum, plasma, cell culture media etc
SpeciesAll
Protocol Length20 min
Size100 tests
Shelf Life6 months

More Details

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.
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