L- Lactate Assay Kit - Information
Assay Genie's colorimetric L- lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.
For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.
L- Lactate Assay Kit- Key Features
- Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
L- Lactate Assay Kit - Data Sheet
|Method of Detection||OD565nm|
|Detection Limit||0.05 mM|
|Samples||Serum, plasma, cell culture media etc|
|Protocol Length||20 min|
|Shelf Life||6 months|
is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.