Anti-FHL2 Antibody (CAB1907)

Antibody Name:	Anti-FHL2 Antibody
Antibody SKU:	CAB1907
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-279 of human FHL2 (NP_001034581.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	ES-2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-279 of human FHL2 (NP_001034581.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTER FDCH HCNE SLFG KKYI LREE SPYC VVCF ETLF ANTC EECG KPIG CDCK DLSY KDRH WHEA CFHC SQCR NSLV DKPF AAKE DQLL CTDC YSNE YSSK CQEC KKTI MPGT RKME YKGS SWHE TCFI CHRC QQPI GTKS FIPK DNQN FCVP CYEK QHAM QCVQ CKKP ITTG GVTY REQP WHKE CFVC TACR KQLS GQRF TARD DFAY CLNC FCDL YAKK CAGC TNPI SGLG GTKY ISFE ERQW HNDC FNCK KCSL SLVG RGFL TERD DILC PDCG KDI
Gene ID:	2274
Uniprot:	Q14192
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	15kDa/32kDa
Observed MW:	32kDa

Synonyms:

FHL2, AAG11, DRAL, FHL-2, SLIM-3, SLIM3

Background:

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified.

UniProt Protein Function:	FHL2: May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 2q12.2 Cellular Component: nucleoplasm; focal adhesion; M band; nucleus; Z disc; actin cytoskeleton Molecular Function:identical protein binding; protein binding; androgen receptor binding; zinc ion binding; transcription coactivator activity; transcription factor binding Biological Process: osteoblast differentiation; atrial cardiac muscle cell development; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell development; androgen receptor signaling pathway; response to hormone stimulus; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of apoptosis
NCBI Summary:	This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2016]
UniProt Code:	Q14192
NCBI GenInfo Identifier:	116241364
NCBI Gene ID:	2274
NCBI Accession:	Q14192.3
UniProt Related Accession:	Q14192
Molecular Weight:	~ 32kDa
NCBI Full Name:	Four and a half LIM domains protein 2
NCBI Synonym Full Names:	four and a half LIM domains 2
NCBI Official Symbol:	FHL2
NCBI Official Synonym Symbols:	DRAL; AAG11; FHL-2; SLIM3; SLIM-3
NCBI Protein Information:	four and a half LIM domains protein 2
UniProt Protein Name:	Four and a half LIM domains protein 2
UniProt Synonym Protein Names:	LIM domain protein DRAL; Skeletal muscle LIM-protein 3; SLIM-3
Protein Family:	Four and a half LIM domains protein
UniProt Gene Name:	FHL2
UniProt Entry Name:	FHL2_HUMAN

	Western blot analysis of extracts of ES-2 cells, using FHL2 antibody (CAB1907) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021).
	Immunohistochemistry of paraffin-embedded rat kidney using FHL2 antibody (CAB1907) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using FHL2 antibody (CAB1907) at dilution of 1:100 (40x lens).

Antibodies

FHL2 Antibody (PACO09296)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)