The FITC Anti-Mouse LY6A/E (Sca-1) Antibody (D7) offered by Assay Genie is a valuable tool for researchers studying mouse models and exploring the role of LY6A/E (Sca-1), a cell surface marker associated with stem cell and immune cell populations. This monoclonal antibody, designated D7, is specifically designed to target LY6A/E (Sca-1) in mouse samples, allowing for precise detection and analysis in flow cytometry and immunofluorescence applications.LY6A/E (Sca-1) is a well-known marker for stem cells, particularly hematopoietic stem cells, as well as various immune cell subsets. By targeting LY6A/E (Sca-1), researchers can gain insights into cell populations, differentiation processes, and immune responses in experimental settings.
The FITC conjugation of this antibody enables fluorescence-based detection, making it a versatile tool for multi-color flow cytometry experiments and visualization of LY6A/E (Sca-1)-expressing cells in tissues and cell cultures.The D7 FITC Anti-Mouse LY6A/E (Sca-1) Antibody from Assay Genie provides reliable and specific detection of LY6A/E (Sca-1) in mouse samples, offering researchers a valuable resource for characterizing cell populations and studying cellular dynamics in various research fields, including stem cell biology, immunology, and developmental biology.
Product Title:
FITC Anti-Mouse Ly6A/E (Sca-1) Antibody [D7]
SKU:
AGEL2345
Size:
50 Tests, 100 Tests, 200 Tests
Clone No:
D7
Synonyms:
Ly6a;Ly6;Ly-6A.2/Ly-6E.1;SCA-1;TAP;
Host Species:
Rat
Reactivity:
Mouse
Application:
FCM
Isotype:
Rat IgG2a, κ
Isotype Control:
FITC Rat IgG2a, κ Isotype Control[2A3]
Form:
Liquid
Conjugation:
FITC
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P05533
Gene ID:
110454
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.
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