Description
Gallus Cytokine Array (10 targets) (SARB0014)
The Gallus Cytokine Array 10 (SARB0014) is a powerful tool for studying cytokine expression in avian species, specifically chickens. This array allows for the simultaneous detection of 10 different cytokines, including interleukins, interferons, and growth factors, in a single sample. Utilizing advanced antibody-based technology, the Gallus Cytokine Array 10 provides researchers with a comprehensive analysis of the immune response in chickens, aiding in the understanding of immune regulation, inflammation, and disease pathogenesis.
With its high sensitivity and specificity, this cytokine array is ideal for studies in poultry health, infectious diseases, and vaccine development. The Gallus Cytokine Array 10 is a valuable tool for researchers looking to unravel the complexities of avian immunology and advance our knowledge of host-pathogen interactions in poultry species.
| Product Sku: | SARB0014 |
| Size: | 2, 4, or 8 |
| Species Detected: | Gallus |
| Number of Targets Detected: | 10 |
| Gene Symbols: | CAR, CCL5, IFNG, IL10, IL12B, IL16, IL21, IL6, NTN2, NTN3|PTX3, TNFAIP5, TSG14 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Caronte
| IFN-gamma
| IL-6
| IL-10
| IL-12 p40
|
IL-16
| IL-21
| Netrin-2
| Pentraxin-3 (TSG-14)
| RANTES (CCL5)
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Gallus (Chicken) Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
