Anti-GANAB Antibody (CAB13851)

Antibody Name:	Anti-GANAB Antibody
Antibody SKU:	CAB13851
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GANAB (NP_001265123.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, Raji, HepG2, HeLa, Mouse brain, Mouse liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GANAB (NP_001265123.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTRF RIDE LEPR RPRY RVPD VLVA DPPI ARLS VSGR DENS VELT MAEG PYKI ILTA RPFR LDLL EDRS LLLS VNAR GLLE FEHQ RAPR VSQG SKDP AEGD GAQP EETP RDGD KPEE TQGK AEKD EPGA WEET FKTH SDSK PYGP MSVG LDFS LPGM EHVY GIPE HADN LRLK VTEG GEPY RLYN LDVF QYEL YNPM ALYG SVPV LLAH NPHR DLGI FWLN AAET WVDI SSNT AGKT LFGK MMDY LQGS GETP QTDV RWMS
Gene ID:	23193
Uniprot:	Q14697
Cellular Location:	Endoplasmic reticulum, Golgi apparatus, Melanosome
Calculated MW:	5kDa/106kDa/109kDa
Observed MW:	112kDa

Synonyms:

GANAB, G2AN, GIIA, GLUII, PKD3

Background:

This gene encodes the alpha subunit of glucosidase II and a member of the glycosyl hydrolase 31 family of proteins. The heterodimeric enzyme glucosidase II plays a role in protein folding and quality control by cleaving glucose residues from immature glycoproteins in the endoplasmic reticulum. Expression of the encoded protein is elevated in lung tumor tissue and in response to UV irradiation. Mutations in this gene cause autosomal-dominant polycystic kidney and liver disease.

UniProt Code:	Q14697
NCBI GenInfo Identifier:	38202257
NCBI Gene ID:	23193
NCBI Accession:	NP_938148
UniProt Secondary Accession:	Q14697,Q8WTS9, Q9P0X0, A6NC20,
UniProt Related Accession:	Q14697
Molecular Weight:	5,673 Da
NCBI Full Name:	neutral alpha-glucosidase AB isoform 2
NCBI Synonym Full Names:	glucosidase, alpha; neutral AB
NCBI Official Symbol:	GANAB
NCBI Official Synonym Symbols:	G2AN; GLUII
NCBI Protein Information:	neutral alpha-glucosidase AB; HEL-S-164nA; alpha glucosidase II alpha subunit; alpha-glucosidase 2; epididymis secretory sperm binding protein Li 164nA; glucosidase II subunit alpha
UniProt Protein Name:
Protein Family:	Neutral alpha-glucosidase
UniProt Gene Name:

	Western blot analysis of extracts of various cell lines, using GANAB antibody (CAB13851) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat brain using GANAB antibody (CAB13851) at dilution of 1:150 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using GANAB antibody (CAB13851) at dilution of 1:150 (40x lens).
	Immunofluorescence analysis of C6 cells using GANAB antibody (CAB13851) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using GANAB antibody (CAB13851) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-GANAB Antibody (CAB19876)[KO Validated]

GANAB Antibody (PACO02759)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)