GeniePlex Non-Human Primate IGF I and IGFII 2-Plex (96 Tests)



ELISA Kit Technical Manual

Product Name: Genieplex NHP IGF I and IGF II 2-plex Immunoassay Panel



Species Reactivity:

Non-Human Primate


Assay Type:


Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

This GeniePlex Genieplex NHP IGF I and IGF II 2-plex Immunoassay Panel is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IGF I and IGF II on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 2 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Product Information

Reactivity: Non-Human Primate
Analytes: IGF I and IGF II
1x Ab-conjugated beads (premixed): S4P5 - anti-NHP IGF I; S5P8 - anti-NHP IGF II
2x Biotin-detection Ab (premixed): Biotin - anti-NHP IGF I; Biotin - anti-NHP IGF II
Buffers: 2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix: Lyophilized Standard Mix-NHP IGF I and IGF II, 2-Plex. One vial containing lyophilized recombinant IGF I and IGF II.
Sample types: Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs): IGF I, < 10 pg/mL; IGF II, < 10 pg/mL.
LLOQs: IGF I, > 20 pg/mL; IGF II, < 20 pg/mL.
ULOQs: IGF I, < 20,000 pg/mL; IGF II, <20,000 pg/mL
Standard dose recovery: Standard dose recovery: 70-130%
Intra-assay CV: Intra-assay CV: < 10%
Inter-assay CV: Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Cross-reactivity: IGF I: Negligible cross-reactivity with IGF II at 10,000pg/mL IGF II: < 1% cross-reactivity with IGF I at 10,000pg/mL
Sample volume: Sample volume: 15 µL/test


ELISA Kit Technical Manual

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Additional Information

Product type:
Non-Human Primate
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