GeniePlex Non-human Primate (NHP) Th1/Th2/Th17 7-Plex (96 Tests)

(No reviews yet) Write a Review
SKU:
MKAMPM002
€1,326

Description

ELISA Kit Technical Manual

Product Name: Genieplex NHP Th1/Th2/Th17 7-plex Kit

Analytes

IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha

Species Reactivity:

Non-Human Primate

 

Assay Type:

Multiplex

Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

This GeniePlex Genieplex NHP Th1/Th2/Th17 7-plex Kit is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 7 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Product Information

Reactivity: Non-Human Primate
Analytes: IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha
1x Ab-conjugated beads (premixed): S4P3 - anti-NHP TNF-Alpha; S4P7 - anti-NHP IL-2; S4P9 - anti-NHP IFN-Gamma; S5P9 - anti-NHP IL-17A; S5P4 - anti-NHP IL-4; S5P6 - anti-NHP IL-6; S5P10 - anti-NHP IL-10
2x Biotin-detection Ab (premixed): Biotin - anti-NHP TNF-Alpha; Biotin - anti-NHP IL-2; Biotin - anti -NHP IFN-Gamma; Biotin - anti-NHP IL-17A; Biotin - anti-NHP IL-4; Biotin - anti-NHP IL-6; Biotin - anti-NHP IL-10
Buffers: 2x Mouse/Rat dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix: Lyophilized Standard Mix-NHP Group 1 Panel A, 7-Plex. One vial containing lyophilized recombinant IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha.
Sample types: Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs): IFN-Gamma, < 5 pg/mL; IL-2, < 5 pg/mL; IL-4 < 1 pg/mL, IL-6 LLOQs: IFN-Gamma, < 10 pg/mL; IL-2, < 10 pg/mL; IL-4 < 5 pg/mL, IL-6 ULOQs: IFN-Gamma, > 5,000 pg/mL; IL-2, > 5,000 pg/mL; IL-4 > 2,000 pg/mL; IL-6 > 5,000 pg/ml; IL-10, > 5,000 pg/mL; IL-17A, > 5,000 pg/mL; TNF-Alpha, > 5,000 pg/mL. Standard dose recovery: Standard dose recovery: 70-130% Intra-assay CV: Intra-assay CV: < 10% Inter-assay CV: Inter-assay CV: < 20% Cross-reactivity of analytes in the Panel: Cross-reactivity of analytes in the Panel: Negligible Sample volume: Sample volume: 15 µL/test

Datasheets

ELISA Kit Technical Manual

View AllClose

Additional Information

Product type:
Multiplex
Reactivity:
Non-Human Primate
ELISA Type:
Multiplex
View AllClose

0 Reviews

View AllClose