The GOT1 Monoclonal Antibody (CAB11363) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbit monoclonal technology, offers high specificity and sensitivity for detecting GOT1 in various samples, particularly human tissues.GOT1 plays a crucial role in the conversion of aspartate to oxaloacetate, a key step in the urea cycle and amino acid metabolism. Dysregulation of GOT1 has been implicated in various diseases, including cancer and metabolic disorders, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GOT1 Monoclonal Antibody
SKU:
CAB11363
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0579
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Hep G2, A549, U-87MG, Mouse liver, Mouse brain, Mouse heart, Rat liver, Rat brain, Rat kidney
Cellular Localization:
Cytoplasm.
Calculated MW:
46kDa
Observed MW:
41kDa
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Purification Method
Affinity purification
Gene ID
2805
RRID
AB_2861551
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GOT1 Rabbit mAb (CAB11363) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using GOT1 Rabbit mAb (CAB11363) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
