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GOT1 Rabbit Monoclonal Antibody (CAB11363)

SKU:
CAB11363
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Metabolism
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:GOT1 Rabbit Monoclonal Antibody
SKU:CAB11363
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human GOT1 (NP_002070.1).
Sequence:AAGF KDIR SYRY WDAE KRGL DLQG FLND LENA PEFS IVVL HACA HNPT GIDP TPEQ WKQI ASVM KHRF LFPF FDSA YQGF ASGN LERD AWAI RYFV SEGF E
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:AST; AST1; SGOT; cCAT; GIG18; cAspAT; ASTQTL1; GOT1
Positive Sample:Hep G2,A549,U-87MG,Mouse liver,Mouse brain,Mouse heart,Rat liver,Rat brain,Rat kidney
Conjugate:Unconjugated
Cellular Localization:Cytoplasm
Calculated MW:46kDa
Observed MW:41kDa

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Purification Method:Affinity purification
Gene ID:2805
Clone Number:ARC0579
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using GOT1 Rabbit mAb (CAB11363) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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